Cannsep A is a reverse-phase analytical column with embedded strong acidic ion-pairing groups.
This stationary phase retains neutral cannabinoids through a reverse-phase mechanism. Acidic cannabinoids, such as Tetrahydrocannabinolic acid (THCA) and Cannabidiolic acid (CBDA), will be subject to ion-exclusion interactions with the negatively charged stationary phase of Cannsep A.
Mobile phases can be selected for compatibility with low UV detection, using phosphoric or sulfuric acid as modifiers. Alternatively, LC-MS and preparative chromatography compatible mobile phases can be used with formic acid as a modifier.
