Obelisc N is a mixed-mode column characterized by its highly polar properties, making it well-suited for separating polar and charged analytes.
– Obelisc N features both positively and negatively charged groups, allowing it to interact with analytes that are either positively or negatively charged.
– The column supports multiple separation modes, including normal phase (NP), hydrophilic interaction liquid chromatography (HILIC), and ion exclusion (IE).
– In HILIC mode, Obelisc N displays different selectivity compared to other HILIC or bare silica columns.
– The column is compatible with liquid chromatography-mass spectrometry (LC-MS), evaporative light scattering detection (ELSD), preparative chromatography, and low ultraviolet (UV) detection below 220 nm, using low concentration buffers.