YMC-Guardpack Ph 50 mm x 50.0 mm x 5 um

Unique Selectivity

Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed-phase materials such as C18, C8, and C4.  The π electrons of the phenyl group can interact with the aromatic ring(s) of an analyte to increase retention relative to non-aromatic species.  Phenyl also has application as an alternative to C18 and C4 for the analysis of peptides and proteins on both small-pore (120Å) and wide-pore (300Å) silica supports.  Retention is decreased on wide-pore phenyl phases relative to 120Å material.  Generally, as pore size increases, surface area decreases, resulting in shorter retention times.

YMC-Pack Ph produces different separation characteristics from alkyl-silica stationary phases (C18, C8, C4) for analytes with aromatic rings due to the π – π and hydrophobic interactions contributing to the separation.

• Monomerically-bonded phenyl
• Fully endcapped
• Preferential retention of aromatic compounds
• Alternative to alkyl-bonded phases for the analysis of peptides and other biomolecules